5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
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Facts-enriched characterization of cell populations. Morphological info from pictures can include into the richness of flow cytometry facts. As an example, the determine displays an or else conventional apoptosis assay applying Annexin V and PI, introducing cell imaging to characterize cells in Every single inhabitants to expose morphologically distinctive attributes. These insights could not have been gained from multiplex staining by itself.
No matter if you’re a newbie or a professional, examine our means to advance your study and expertise in flow cytometry.
Mix acoustic concentrating know-how with substantial-parameter analysis for flexible spectral unmixing and traditional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating that has a large-velocity brightfield camera for simultaneous large throughput flow cytometry and substantial resolution brightfield imaging.
"A picture isn't really very well worth one thousand dots, nevertheless it genuinely really helps to see images. It is great to determine images that correlate with staining. By way of example, AnnexinV-favourable cells which might be morphologically diverse than healthful cells, or CD14+ monocytes that are greater plus more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it really is illuminating to discover what number of doublets slip by means of traditional singlet gates, and fantastic to verify the status of unforeseen double-beneficial gatherings in my Examination."
Pair your Attune flow cytometer with an autosampler for increased workflow efficiency, and insert robotic integration for maximum throughput.
Our experts will style panels for you personally as persistently you will need—cost-free, no merchandise acquire desired.
Early indications of this contamination had originally been dismissed as particles. Backgating to discover new populations. Backgating imaged cells about check here the Attune CytPix Flow Cytometer also allows you to use morphological functions to find out exciting subpopulations that may not be clear from flow cytometry info by yourself.
Most likely most importantly, these activities contain cells of clearly various phenotypes which could lead to incorrect conclusions about double favourable events (especially in rare populations).
AI-pushed automated image Assessment Together with the Attune Cytometric Software interprets occasion characteristics into distinct morphology parameters that could be coupled with conventional fluorescence and scatter parameters to reveal extremely reproducible population adjustments and distinctive subsets.
We previously shown the strength of imaging CAR-T/Ramos mobile interactions. Allow’s examine just the population of best interest, the double good events, To find out more. We will now use prolonged morphological parameters (right here, circularity and skewness of depth) to further take a look at the functions of such populations and refine gating on these occasions, raising details robustness.
Leverage the power of Superior technology and innovation to speed up your discoveries. Have faith in inside the Attune Flow Cytometer relatives to provide the trustworthiness, velocity, and precision that established your work apart.
No clean/no lyse leukocyte Investigation with imaging. Cells ended up obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Pro UX/UI permits buyers to educate AI designs for expanded programs. (A) The “Practice Model” attribute offers annotation instruments and actual-time model schooling accuracy.
Gating on these extended parameters means that you can quickly and correctly detect populations of interest and make sure gating strategy with little if any manual critique. The Attune software package delivers entry to point out-of-the-art equipment Mastering Evaluation resources in just an expertly made user interface, making it extremely user friendly.
As the cell line had previously been analyzed to the Attune CytPix Flow Cytometer, the scientists went again to the pictures and have been ready to document the microbial an infection not less than five days before.
Agilent Value Guarantee guarantees you a minimum of 10 years of instrument use through the day you buy. Usually, we will credit you Using the residual value of the program towards an upgraded design.
Here, we demonstrate that by gating methods making use of impression-dependent quantitative parameters can distinguish interacting cells from coincident functions to more correctly examine interacting cells.